Sintesis dan Karakterisasi Nanokomposit Hidroksiapatit/Tembaga Oksida Sebagai Antibakteri Escherchia coli

Yuanita Amalia Hariyanto, M Helmi Hakim, Ratika Sekar Ajeng Ananingtyas, Anindya Bella Monica

Abstract


Pengolahan limbah cangkang Achatina Fulica yang dimanfaatkan sebagai bahan dasar utama pembuatan hydroxyapatite (HAp). Dalam upaya  menjaga perfomanya, HAp dikompositkan dengan material yang memiliki kemampuan khusus yang sama dan biokompatibilitasnya sangat baik yaitu CuO. Setelah dilakukan komposit material, hasil dari sintesis tersebut dimasukkan ke tahap karakterisasi menggunakan XRD menunjukkan HAp muncul pada = 25,9o; 31,9o; 39,57o; 46,62o, 49,47o. Untuk puncak CuO terdeteksi pada 2-teta = 35,544o, 38,709o, 48,717o, 58,265, dan 61,526o. Selanjutnya, untuk data hasil FTIR menunjukkan terdeteksinya puncak Cu-O berada dalam kisaran 400 hingga 600 cm-1. Untuk puncak Hap terdeteksi gugus fungsi fosfat (PO43) pada kisaran 560, 618, 987, dan 1060 cm-1. Sedangkan fungsi karbonat (CO32-) pada kisaran 910 cm-1 dan 1630-1670 cm-1. Berdasarkan aktivitas antimikroba, pada nanokomposit Hap/CuO menunjukkan kemampuan yang baik dalam membunuh koloni bakteri yang sangat baik.

Keywords


Kalsium Hidroksida; cangkang bekicot; kopresipitasi; partikel

References


Amalia Hariyanto, Y., Taufiq, A., Sunaryono, Mufti, N., Soontaranon, S., & Kamonsutthipaijit, N. (2019). Study on Structural Characters of Nano-sized Hydroxyapatite Prepared from Limestone. IOP Conference Series: Materials Science and Engineering, 515, 012020. https://doi.org/10.1088/1757-899X/515/1/012020

Amiri, M., Etemadifar, Z., Daneshkazemi, A., & Nateghi, M. (2017). Antimicrobial Effect of Copper Oxide Nanoparticles on Some Oral Bacteria and Candida Species. Journal of Dental Biomaterials, 4(1), 347–352.

C. Vanitha, M. R. (2017). Synthesis, Characterization of Nano-Hydroxy Apatite From White Snail Shells and Removal of Methylene Blue. 4.

Charlena, Suparto, I. H., & Kurniawan, E. (2019). Synthesis and Characterization of Hydroxyapatite-Zinc Oxide (HAp-ZnO) as Antibacterial Biomaterial. IOP Conference Series: Materials Science and Engineering, 599, 012011. https://doi.org/10.1088/1757-899X/599/1/012011

Chauhan, M., Sharma, B., Kumar, R., Chaudhary, G. R., Hassan, A. A., & Kumar, S. (2019). Green synthesis of CuO nanomaterials and their proficient use for organic waste removal and antimicrobial application. Environmental Research, 168, 85–95.

https://doi.org/10.1016/j.envres.2018.09.024

Eshed, M., Lellouche, J., Matalon, S., Gedanken, A., & Banin, E. (2012). Sonochemical Coatings of ZnO and CuO Nanoparticles Inhibit Streptococcus mutans Biofilm Formation on Teeth Model. Langmuir, 28(33), 12288–12295. https://doi.org/10.1021/la301432a

Fajri, H. (2016). Synthesize and Characterization of Hydroxyapatite from Freshwater Snail Shell Sulcospira Sp. Proceed by Combination of Ball Milling and Heat Treatment. 37, 9–14.

Feng, L., Wang, R., Zhang, Y., Ji, S., Chuan, Y., Zhang, W., Liu, B., Yuan, C., & Du, C. (2019). In situ XRD observation of CuO anode phase conversion in lithium-ion batteries. Journal of Materials Science, 54(2), 1520–1528. https://doi.org/10.1007/s10853-018-2885-0

Hariyanto, Y. A. (2018). Investigasi nanostruktur 3-dimensi hidroksiapatit/magnetit dari bahan alam menggunakan metode SAXS dan karakterisasi sifat toksik [PhD Thesis]. Universitas Negeri Malang.

Hariyanto, Y. A., Taufiq, A., Mufti, N., Soontaranon, S., & Kamonsutthipaijit, N. (2019). Study on Structural Characters of Nano-sized Hydroxyapatite Prepared from Limestone. IOP Conference Series: Materials Science and Engineering, 515, 012020.

Hariyanto, Y. A., Taufiq, A., Sunaryono, & Soontaranon, S. (2019). Investigation on the Three-Dimensional Nanostructure and the Optical Properties of Hydroxyapatite/Magnetite Nanocomposites Prepared from Natural Resources. Journal of the Korean Physical Society, 75(9), 708–715. https://doi.org/10.3938/jkps.75.708

Khashan, K. S., Sulaiman, G. M., & Abdulameer, F. A. (2016). Synthesis and Antibacterial Activity of CuO Nanoparticles Suspension Induced by Laser Ablation in Liquid. Arabian Journal for Science and Engineering, 41(1), 301–310. https://doi.org/10.1007/s13369-015-1733-7

Khuluqi, M. H., Prapdito, R. R., & Sambodo, F. P. (2018). Prediction accident triangle in maintenance of underground mine facilities using Poisson distribution analysis. IOP Conference Series: Materials Science and Engineering, 337, 012069. https://doi.org/10.1088/1757-899X/337/1/012069

Kim, J.-H., Cho, H., Ryu, S.-E., & Choi, M.-U. (2000). Effects of Metal Ions on the Activity of Protein Tyrosine Phosphatase VHR: Highly Potent and Reversible Oxidative Inactivation by Cu2+ Ion. Archives of Biochemistry and Biophysics, 382(1), 72–80. https://doi.org/10.1006/abbi.2000.1996

Kumar, G. S., Sathish, L., Govindan, R., & Girija, E. K. (2015). Utilization of snail shells to synthesise hydroxyapatite nanorods for orthopedic applications. RSC Advances, 5(49), 39544–39548. https://doi.org/10.1039/C5RA04402B

Lopes, M. A., Monteiro, F. J., & Santos, J. D. (1999). Glass-reinforced hydroxyapatite composites: Fracture toughness and hardness dependence on microstructural characteristics. Biomaterials, 20(21), 2085–2090. https://doi.org/10.1016/S0142-9612(99)00112-X

Mahartha, G. R. A. (2013). MANAGEMENT OF FRACTURE OF MUSCULOSCELETAL TRAUMA.

Maifita, Y., . Z., & Oktafiyanti, R. (2020). THE EFFECT OF GIVING EEL FLOUR (MONOPTERUS ALBUS) WITHLEVELS TO THE ACCELERATION OF FRACTURE HEALING PROCESSAT RATTUS NOVERGICUS WISTAR ALBINO JANTAN. Malaysian Journal of Medical Research, 4(4). https://doi.org/10.31674/mjmr.2020.v04i04.008

Pandiyarajan, T., Udayabhaskar, R., Vignesh, S., James, R. A., & Karthikeyan, B. (2013). Synthesis and concentration dependent antibacterial activities of CuO nanoflakes. Materials Science and Engineering: C, 33(4), 2020–2024. https://doi.org/10.1016/j.msec.2013.01.021

Prabhu, Y. T., Rao, K. V., Kumari, B. S., Kumar, V. S. S., & Pavani, T. (2015). Synthesis of Fe 3 O 4 nanoparticles and its antibacterial application. International Nano Letters, 5(2), 85–92.

Puspitasari, P., Fauzi, A. F., Susanto, H., Permanasari, A. A., Gayatri, R. W., Razak, J. A., & Abdillah Pratama, M. M. (2021). Synthesis and characterization of CaCo 3 /CaO from Achatina fulica in various sintering time. IOP Conference Series: Materials Science and Engineering, 1034(1), 012093. https://doi.org/10.1088/1757-899X/1034/1/012093

Rehman, I., & Bonfield, W. (1997). Characterization of hydroxyapatite and carbonated apatite by photo acoustic FTIR spectroscopy. Journal of Materials Science: Materials in Medicine, 8(1), 1–4. https://doi.org/10.1023/A:1018570213546

Sahmani, S., Saber-Samandari, S., Shahali, M., Joneidi Yekta, H., Aghadavoudi, F., Montazeran, A. H., Aghdam, M. M., & Khandan, A. (2018). Mechanical and biological performance of axially loaded novel bio-nanocomposite sandwich plate-type implant coated by biological polymer thin film. Journal of the Mechanical Behavior of Biomedical Materials, 88, 238–250. https://doi.org/10.1016/j.jmbbm.2018.08.030

Sahmani, S., Shahali, M., Ghadiri Nejad, M., Khandan, A., Aghdam, M. M., & Saber-Samandari, S. (2019). Effect of copper oxide nanoparticles on electrical conductivity and cell viability of calcium phosphate scaffolds with improved mechanical strength for bone tissue engineering. The European Physical Journal Plus, 134(1), 7. https://doi.org/10.1140/epjp/i2019-12375-x

Santosa, S. P. (2017). Anatomy of injury severity and fatality in Indonesian traffic accidents. 49(3), 412–422.

Saputro, R. E. (2019). Preparation of Fe3O4/OA/DMSO Ferrofluids using a double surfactant system as antifungal materials candidate. IOP Conference Series: Materials Science and Engineering (p. 012029). IOP Publishing.

Shareef, M. Y., Messer, P. F., & van Noort, R. (1993). Fabrication, characterization and fracture study of a machinable hydroxyapatite ceramic. Biomaterials, 14(1), 69–75. https://doi.org/10.1016/0142-9612(93)90078-G

Sharmila, G., Sakthi Pradeep, R., Sandiya, K., Santhiya, S., Muthukumaran, C., Jeyanthi, J., Manoj Kumar, N., & Thirumarimurugan, M. (2018). Biogenic synthesis of CuO nanoparticles using Bauhinia tomentosa leaves extract: Characterization and its antibacterial application. Journal of Molecular Structure, 1165, 288–292. https://doi.org/10.1016/j.molstruc.2018.04.011

Valizadeh, S., Rasoulifard, M. H., & Dorraji, M. S. S. (2014). Modified Fe3O4- hydroxyapatite nanocomposites as heterogeneous catalysts in three UV, Vis and Fenton like degradation systems. Applied Surface Science, 319, 358–366. https://doi.org/10.1016/j.apsusc.2014.07.139

Ye, D., Tang, W., Xu, Z., Zhao, X., & Wang, G. (2018). Application of MBG as a coating material on mechanically stronger but less degradable ceramic scaffolds for enhanced osteogenesis. Materials Letters, 223, 105–108. https://doi.org/10.1016/j.matlet.2018.03.202




DOI: http://dx.doi.org/10.28926/briliant.v7i2.885

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Briliant: Jurnal Riset dan Konseptual

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

Published by:

Lembaga Penelitian dan Pengabdian Masyarakat

Universitas Nahdlatul Ulama Blitar